Blocking Phosphotyrosine Blots

[sam simons]

5% dry milk in PBS with 0.1% Tween-20 is a sufficient block. For 
chemical detection overnight in the cold is OK but for ECL it will give 
you that background. You shouldn't need to block more than 20 minutes at 
room temp. Of course you then have to do the entire procedure in one 
day, but you will think its worth it when you see the result!

Happy Protein Hunting

Sam


The opinions expressed here are my own blah, blah, blah