Supplier for secondary antibody goat anti-mouse

Harry J. Witchel Harry.Witchel at bristol.ac.uk
Thu Feb 1 13:39:10 EST 1996


Felicitations molecular masters of the Fates --
	My friend Marcelo is having a high background problem with his primary 
expression screen which he suspects is due to his secondary antibody.  He is 
expressing in pSPORT and most colonies (bug library) have a fair bit of 
background.  When he does the westerns, he gets several bands (all faint) in 
the IPTG induced lane, but he expects only one band as the antibody is a 
monoclonal raised against a mammalian differentiated protein (normally 
expressed probably only in the cochlea).  He suspects that his secondary 
antibody (Sigma, goat anti-mouse affinity purified) may be picking up some of 
his bug proteins (he is running the secondary-only Westerns now).
	Assuming his secondary antibody is picking up bug stuff, which 
suppliers (UK or international) sell the really fine secondary antibodies. 
	Surf in peace
	Harry

Harry.Witchel at Bristol.Ac.UK




More information about the Methods mailing list