Lambda Screening Problems
Harry J. Witchel
Harry.Witchel at bristol.ac.uk
Thu Feb 1 13:20:17 EST 1996
I would be keen if you could forward the answers you get to me as
Harry.Witchel at Bristol.Ac.UK
In article <4ej142$324 at mserv1.dl.ac.uk>, DOCON at ollamh.ucd.ie says...
I have identified strong positive
>primary plaques when first screening 300,000 pfu's, around 10-12 such
>positives, all duplicating nicely with the duplicate scren.
>The problem lies in the secondary screen of the plugged primaries.
>These have been plated out at high and low density on 150mm plates
>and lifted and probed with the same probe showing good specific
>activity etc.. None of these primaries has been supported by the
>secondary screen, i.e., all negative.
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