Lambda Screening Problems

Harry J. Witchel Harry.Witchel at bristol.ac.uk
Thu Feb 1 13:20:17 EST 1996


Dave --
	I would be keen if you could forward the answers you get to me as 
well.
	Many thanks
	Harry

Harry.Witchel at Bristol.Ac.UK


In article <4ej142$324 at mserv1.dl.ac.uk>, DOCON at ollamh.ucd.ie says...
>
 I have identified strong positive 
>primary plaques when first screening 300,000 pfu's, around 10-12 such 
>positives, all duplicating nicely with the duplicate scren.
>The problem lies in the secondary screen of the plugged primaries. 
>These have been plated out at high and low density on 150mm plates 
>and lifted and probed with the same probe showing good specific 
>activity etc.. None of these primaries has been supported by the 
>secondary screen, i.e., all negative. 
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