Taq error "spectrum"

Bernard Murray bernard at elsie.nci.nih.gov
Thu Feb 1 15:19:58 EST 1996


Hello all,

	I was just wondering if anyone knew of a study looking at the
types of errors introduced by Taq during PCR.  I realise that substitutions
are more common than frameshifts but am not aware of any looking at Taq's
"preference" for actual substitutions.  Does the spectrum vary
qualitatively and/or quantitatively with the sequence context (eg. high
%GC).
	Any pointers gratefully received,
			Bernard
Bernard Murray, Ph.D.
bernard at elsie.nci.nih.gov  (National Cancer Institute, NIH, Bethesda MD, USA)




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