Ligation problems on agarose-isolated fragments
SaiSu at MCB.LiU.SE
Thu Feb 1 13:07:18 EST 1996
OH! This is pretty simple, all you have to do is extensively
digest your insert and vectors with the designated restriction
enzymes and then gel purify them, we have found that quiaex for
this purpose to be very good.
Then ligate them using the normal procedures of what ever you
have been using, but after the ligation, clean the plasmid by
EtOH ppt. The ppt can be dissolved in TE or DW and used for
Works very good, if you have still problems, pls dont hesitate
to contact me.
As I sail far far away...
Inst for Cell Biologi
Linkoping. S 581 85
Tele :+46-13-223917 (Res :+46-13-138839)
E.mail: Saisu at mcb.liu.se
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