Ligation problems on agarose-isolated fragments

Dr.Sailesh Surapureddi SaiSu at MCB.LiU.SE
Thu Feb 1 13:07:18 EST 1996


OH! This is pretty simple, all you have to do is extensively 
digest your insert and vectors with the designated restriction 
enzymes and then gel purify them, we have found that quiaex for 
this purpose to be very good.
		Then ligate them using the normal procedures of what ever you 
have been using, but after the ligation, clean the plasmid by 
EtOH ppt. The ppt can be dissolved in TE or DW and used for 
transformations.

		Works very good, if you have still problems, pls dont hesitate 
to contact me.

Happy ligations.

Sailesh.
       
												As I sail far far away...

Snail Mail:		
Dr.Sailesh Surapureddi	
Post Doc																					
Inst for Cell Biologi							
Halsouniversitetet										
Linkoping. S 581 85										
Sweden.																						
Tele		:+46-13-223917				(Res		:+46-13-138839)
Fax			:+46-13-224149
E.mail: Saisu at mcb.liu.se



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