anti CAT antibody???
Fri Feb 2 09:16:46 EST 1996
In article <4era2b$atp at netnews.upenn.edu>, gdoerre at mail1.sas.upenn.edu
(Geoffrey Doerre) wrote:
> i need an antibody to chloramphenicol acetyltransferase, to look at the
> spatial distribution pattern of my reporter gene expression in the
> context of a whole mount embryo. but i'm having trouble finding a
> commercial source.
> so far i've looked at biodesign, santa cruz, the binding site, and
> sigma. no luck. does anyone have any other ideas? if you could, please
> email any response, 'cause i don't get on the usenet too often. thanks,
> geoff Geoffrey Doerre
> Molecular Biology Grad
> University of Pennsylvania
The best source of CAT antibody I've found is from 5'-3' in Boulder, CO.,
catalogue # 7-310127. It works pretty well on fixed, cultured primary
cells but seems to require a fairly robust promoter to see results. We
haven't tried it on sections but I suppose that the above caveat applies
even more. Hope this helps and happy staining!
sscherer at helix.nih.gov
More information about the Methods