Cell lysates for bFGF-How?

N.Ambalavanan MD Pedp043 at uabdpo.dpo.uab.edu
Sat Feb 3 10:28:49 EST 1996


Hi! I plan to estimate bFGF by ELISA from conditioned media and cell 
lysates of smooth muscle cells grown in 75 cm2 flasks. Does anyone have 
a good protocol for making cell lysates suitable for ELISA? Could you 
please email me a protocol or tell me where to find a good one?
Thanks!



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