Which is the best Method for Purifying DNA for FISH analysis??

Graham Dellaire popa0206 at PO-Box.McGill.CA
Sat Feb 3 16:23:15 EST 1996



I am involved in a project in which I visualize plasmid sequences introduced into the genome of the mouse.


I need a good (re: easy and cheap) protocol for cleaning up my alkaline lysis preps of the plasmid so I can make 
a good probe.


Does any one out there have experience with DNA probes for FISH analysis?  IF you have I would like to hear
how you purified your DNA for your probe....(nick translation with biotin or avidin)


Thanks in advance 


Graham Dellaire
DIvison of Exp. Medicine
McGill University

dellaire at odyssee.net
popa0206 at po-box.mcgill.ca




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