Which is the best Method for Purifying DNA for FISH analysis??
popa0206 at PO-Box.McGill.CA
Sat Feb 3 16:23:15 EST 1996
I am involved in a project in which I visualize plasmid sequences introduced into the genome of the mouse.
I need a good (re: easy and cheap) protocol for cleaning up my alkaline lysis preps of the plasmid so I can make
a good probe.
Does any one out there have experience with DNA probes for FISH analysis? IF you have I would like to hear
how you purified your DNA for your probe....(nick translation with biotin or avidin)
Thanks in advance
DIvison of Exp. Medicine
dellaire at odyssee.net
popa0206 at po-box.mcgill.ca
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