mRNA isolation in E. coli

Rafael Maldonado rafael at
Fri Feb 2 13:34:56 EST 1996

Hi netters!

I need a good, reliable protocol for RNA isolation in E. coli. Since I 
am interested in a time course of amount of mRNA in a culture, it would 
be ideal a method which used low amount of cells (1 ml) to get enough 
mRNA for a primer extension reaction (i. e. 10-40 ug). Any suggestions 



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