Isolation of RNA from wood
hamburg at sage.cc.purdue.edu
Sun Feb 4 13:59:07 EST 1996
I am interested in the isolation of functional RNA from the xylem of red
osier dogwood (Cornus sericea L.) twigs. Only the youngest twigs of
which the maximum diameter is 0.3 inches will be used.
I would be extremely interested to obtain information regarding the
I.) Optimizing the total amount of extractable RNA (cell breakage)
a.) What is the best way to pretreat (cut, rasp) the wood before it
is frozen and stored?
b.) How is the mechanical cell breakage prior to/at the beginning of
the extraction process to be accomplished most efficiently?
c.) Is it possible to isolate functional RNA (in acceptable amounts)
from material that has been freeze-dried (and is therefore easy
II.) Optimizing the quality of the isolated RNA
What potential problems regarding the extraction process itself
have to be taken into account when RNA is extracted from woody
Thanks for your help.
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