Stripping of northern membranes

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Sun Feb 4 22:41:16 EST 1996


Olle Vidal wrote:
> 
> I am currently working with Northern-blots and I am having some trouble with the
> stripping of my hybond-N membranes from the RNA-probes used, a problem which I
> share with others in the lab.  Is there anyone on the net who knows an efficient
> method for getting rid of "sticky" probes.

We boil for 5 minutes in 0.1xSSC, 0.1% SDS...so far everything has come off that we 
have probed with.

Colin



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