Stripping of northern membranes

[Colin Rasmussen]

Olle Vidal wrote:
> 
> I am currently working with Northern-blots and I am having some trouble with the
> stripping of my hybond-N membranes from the RNA-probes used, a problem which I
> share with others in the lab.  Is there anyone on the net who knows an efficient
> method for getting rid of "sticky" probes.

We boil for 5 minutes in 0.1xSSC, 0.1% SDS...so far everything has come off that we 
have probed with.

Colin