Tracking dyes in agarose gel electorphoresis

Ned Mantei mantei at neuro.biol.ethz.ch
Tue Feb 6 01:42:39 EST 1996


In article <4eo5ri$eoq at swen.emba.uvm.edu>, alamarch at moose.uvm.edu
(alamarch) wrote:

> We have used bromophenol blue and xylene cyanol.  We were wondering if
> there were any others dyes which migrated more slowly in a TBE Agarose
> gel

Why more slowly? We use Orange G, which migrates *ahead* (faster) than
even the small DNA fragments. It never happens that a band is obscured by
the tracking dye.

-- 
Ned Mantei
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch   Fax: +41-1-633-1046



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