RT-PCR vs. RNase Protection Assay - Detecting Sense and Antisense

Don Rollins imnr at connectnet.com
Mon Feb 5 12:40:32 EST 1996


Hello all!

What are the advantages and disadvantages of either RT-PCR or RNase
Protection Assay for detecting sense or antisense RNA of the SAME
GENE?

I have cloned hGH into an expression vector in the antisense
orientation, but am still getting secretion of hGH in transfections. I
would like to get an idea of how much bottom strand RNA synthesis I am
getting from my plasmid.

Please post replies directly to this conference, or to the following
e-mail address: 

JOSEGONZ at IMNR.COM

Thanks!

Jose E. N. Gonzales
The Immune Response Corporation




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