Nitrocellulose Phage Lifts-- Reprobing?
The Wagner Lab
ewlab at uci.edu
Mon Feb 5 16:57:32 EST 1996
"Michael W. Thompson" <MTHOM0 at POP.UKY.EDU> wrote:
> I was wondering if anyone knew of a way in which to reprobe old
>filter lifts that were used for genomic cloning? I have about 40 old
>phage lifts which we wish to try to strip and reprobe using a different
>probe. Is there a way to get rid of bound probe and background binding
>without disturbing the bound phage?
Try stripping your filters in 6M Guanidine HCL. It has worked well for
me--no background problems, and only a slight decrease in sensitivity,
but good enough! Have fun.
mdpetros at uci.edu
Molecular Biology and Biochemistry
University of California, Irvine
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