bacterial housekeeping mRNA
crasmussen at anat.med.ualberta.ca
Mon Feb 5 23:36:31 EST 1996
Michael C. Gorry wrote:
> We are interested in testing human fluid samples for
> the presence of bacteria. All we need for now is a
> yes or no answer. Can anyone suggest a good mRNA
> that would be expressed at high levels in bacteria.
> We are looking for something that would be easy
> to amplify using PCR primers. It is our hope to be
> able to detect as many bacterial infections with one
> amplification as possible. I was hoping for some
> housekeeping gene suggestions before having to design
> the primers.
Why not plate out the fluid on nutrient agar and look for colony
formation. Seems easier than PCR.
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