bacterial housekeeping mRNA

Colin Rasmussen crasmussen at
Mon Feb 5 23:36:31 EST 1996

Michael C. Gorry wrote:
> We are interested in testing human fluid samples for
> the presence of bacteria.  All we need for now is a
> yes or no answer.  Can anyone suggest a good mRNA
> that would be expressed at high levels in bacteria.
> We are looking for something that would be easy
> to amplify using PCR primers.  It is our hope to be
> able to detect as many bacterial infections with one
> amplification as possible.  I was hoping for some
> housekeeping gene suggestions before having to design
> the primers.

Why not plate out the fluid on nutrient agar and look for colony 
formation. Seems easier than PCR.

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