bacterial housekeeping mRNA

Colin Rasmussen crasmussen at anat.med.ualberta.ca
Mon Feb 5 23:36:31 EST 1996


Michael C. Gorry wrote:
> 
> We are interested in testing human fluid samples for
> the presence of bacteria.  All we need for now is a
> yes or no answer.  Can anyone suggest a good mRNA
> that would be expressed at high levels in bacteria.
> We are looking for something that would be easy
> to amplify using PCR primers.  It is our hope to be
> able to detect as many bacterial infections with one
> amplification as possible.  I was hoping for some
> housekeeping gene suggestions before having to design
> the primers.
> 

Why not plate out the fluid on nutrient agar and look for colony 
formation. Seems easier than PCR.



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