mung bean nuclease

Anna Blom Anna.Blom at bio.embnet.se
Tue Feb 6 06:42:25 EST 1996


Hello!

I seem to have big problems with Mung Bean Nuclease digestion. It
looks as if it degrades my
double stranded DNA - I just wanted to chew up
the overhang left by
BamHI. I use very little of
enzyme - 100 times less
then recommended by Maniatis, but it still
does not work. My buffer
recipe comes also from Maniatis. Anyone having experience with this
enzyme?



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