mung bean nuclease

Anna Blom Anna.Blom at
Tue Feb 6 06:42:25 EST 1996


I seem to have big problems with Mung Bean Nuclease digestion. It
looks as if it degrades my
double stranded DNA - I just wanted to chew up
the overhang left by
BamHI. I use very little of
enzyme - 100 times less
then recommended by Maniatis, but it still
does not work. My buffer
recipe comes also from Maniatis. Anyone having experience with this

More information about the Methods mailing list