Helper phage, help!!

Nathan Siemers nathan at shiva1-4.bms.com
Wed Feb 7 01:01:51 EST 1996


   > Dear All:
   > 
   >   Any procedure to prepere helper phages stocks? I have some M13k07 (it 
   > has a kanamycin resistance marker) and would like to make more of it.
   > 
   >    Thanks a lot for any help,
   > 
   > 
   >     Vitor Warwar    vwarwar at iq.quim.usp.br

	I believe I just propagated the phage in XL1-blue.  Gave me a
high titer stock that has been used sucessfully to produce ss phagemid
dna.  If there is no other F1 origin to replicate, the phage does
reproduce.


p.s. 5-6 hours, 37 deg C

nathan




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