Helper phage, help!!
nathan at shiva1-4.bms.com
Wed Feb 7 01:01:51 EST 1996
> Dear All:
> Any procedure to prepere helper phages stocks? I have some M13k07 (it
> has a kanamycin resistance marker) and would like to make more of it.
> Thanks a lot for any help,
> Vitor Warwar vwarwar at iq.quim.usp.br
I believe I just propagated the phage in XL1-blue. Gave me a
high titer stock that has been used sucessfully to produce ss phagemid
dna. If there is no other F1 origin to replicate, the phage does
p.s. 5-6 hours, 37 deg C
More information about the Methods