Helper phage, help!!

Nathan Siemers nathan at
Wed Feb 7 01:01:51 EST 1996

   > Dear All:
   >   Any procedure to prepere helper phages stocks? I have some M13k07 (it 
   > has a kanamycin resistance marker) and would like to make more of it.
   >    Thanks a lot for any help,
   >     Vitor Warwar    vwarwar at

	I believe I just propagated the phage in XL1-blue.  Gave me a
high titer stock that has been used sucessfully to produce ss phagemid
dna.  If there is no other F1 origin to replicate, the phage does

p.s. 5-6 hours, 37 deg C


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