Re- Purifying DNA for FISH

Brett Beitzel brett_beitzel at MSMTP.IDDE.SACI.ORG
Tue Feb 6 19:50:14 EST 1996


Subject:Re: Purifying DNA for FISH                 Date: 2/5/96

In my hands, a single phenol:chloroform extraction gives me clean enough DNA
for a nick translation.  I prefer, however, to have primers flanking the
insert on the plasmid and just PCR label the insert.  It seems to work better
than nick translations.

Hope that helps,
Brett Beitzel
brett_beitzel at msmtp.idde.saci.org




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