cDNA synthesis from total RNA
john.brennand at gbapr.zeneca.com
Wed Feb 7 09:27:21 EST 1996
What's known about PolyA+ tails on mRNA of fruit ???
I know that some organisms are either deficient in the activity that
adds the extensions, or produce only short tails.
If this is the case with fruit RNA then you may need to adjust the
conditions for purification.
There are protocols out there (can't recall where) that describe library
production fron total RNA
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