Scaling down DNA synthesis on ABI 381 A ?

Peter Zickert peter.zickert at onkpat.ki.se
Wed Feb 7 05:23:22 EST 1996


I woud like to make 100 different 25-mers for RNA flourescent in-situ
hybridization. 

I have a 381 A with software version 1.23. I wonder if someone have tried
to scale down the synthesis by diluting the phosphoamidites (about 10
times) ?

With 40 nmol scale I should get material for 2000 hybridizations ! 200
would be OK . 

Do I need to upgrade my software to 1.34 or 1.5 ? 

Does anyone have experience with LV columns ?



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