Puromycin Concentration for Selection in Mouse Fibroblasts?

Graham Dellaire popa0206 at PO-Box.McGill.CA
Wed Feb 7 08:57:05 EST 1996


I am using a new Puromycin mammalian expression vector in Mouse L fibroblasts (LTA's).... has anyone worked with these?

I need to know the puromycin concentration to use...

I tried 5, 10 15 and 25 micro grams/ml and they seem to be a little high... except 5 micrograms looks promising.


Graham

dellaire at odyssee.net



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