Biotinylating Peptides

s.mclaren gen136 at
Wed Feb 7 06:33:40 EST 1996


I have been attempting to biotinylate some peptides ( approx.
10 and 22 aa long) using Pierce&Warriner's Biotin-BMCC thro' 
cysteine residues.  The protocol insists on reducing the -SH 
bond using MEA as the initial step - but the reaction needs 
to be cleaned up before proceeding with biotinylation.  

I would like to know if anyone has any advice regarding cleaning 
up the reaction (other than HPLC), and whether this reduction 
is REALLY necessary?

gen136 at

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