Deletion Problems using recombinant PCR
D L Clarke
mb935645 at silver.shef.ac.uk
Wed Feb 7 06:18:21 EST 1996
I would like to create a deletion in gene using recombinant
PCR. I have previously created a restriction site by changing
two base pairs. However, I have been unsuccessful in creating
a deletion. The two primary PCR products can be created and have been
verified. But for the final PCR reaction with the two outside primers
I cannot obtain a product. The two internal primers are 30bp in
length this should be enough to anneal and allow the second
reaction to occur.
I would be most grateful if anybody has any suggestions on the conditions
required to produce the deletion.
D.L.Clarke at Sheffield.ac.uk
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