protein-protein interaction and expression libraries.

[Dr. Jeethan Bendoor]

Dear Joaquim,,
I dont know how many times it has been done, but that is how max (of
myc/max) was cloned a few back years. Check the name Elizabeth Blackwood,
published in Science a few years back, alternatively just do a lit. search
for max, as I guess almost all the papers that mention max, make some kind
of reference to this paper. 
Cheers,
Martin

(lmjakt at hkucc.hku.hk)



In article <jculi-0602962136250001 at powmod.cbm.uam.es>,
jculi at mvax.cbm.uam.es (Joaquim Culi ) wrote:

> Dear netters,
> Do you know how to isolate proteins that interact with another known protein?
> I've been trying the two hybrid system but it hasn't worked very well
> because the piece of my protein that I suspect contains the interaction
> domain also activates transcription. Now I would like to screen a
> expression library in order to find proteins that interact with my protein
> but I don't know any protocol or even if it has been successfully done by
> someone else!!.
> 
> Any protocol or reference will be greatly appreciated.
> 
> Yours,
> 
> Joaquim Culi