Bad message with Ambion Message Machine
phyan at ttuhsc.edu
Wed Feb 7 18:51:23 EST 1996
While most of the time I obtain high yield of a single length message
transcribed with Ambion Kit and then express functional protein in
Xenopus oocytes some time I obtain a smear. This happen with certain
clones and it is not due to RNAse contamination. We tried changing the
ratio cap/gtp, concentration of template and temperature. Absorvance
reading show a ratio of 1.75 to 1.87 and yield in the 19 to 20
micrograms... (The same clone transcribed with Promega give a clean
ban of the right size but the yield is to low and expression in oocytes
Any similar experiences and posible solutions ??
Alan Neely, Ph.D.
Texas Tech U. Health Sciences Center
Lubbock TX, 79430
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