source of lacI repressor wanted

Chris Boyd chrisb at hgu.mrc.ac.uk
Wed Feb 7 11:40:29 EST 1996


Is there a commercial (or other) source of purified lacI repressor
protein?

Even better for us would be a source of the lacI-NLS fusion protein
from the Stratagene LacSwitch system (i.e. as in their plasmid p3'SS).

Failing those, are there any quick (affinity column based?) protocols
for purifying lacI repressor or derivatives in E. coli?

Any information gratefully received.

Best wishes,
--
Chris Boyd          | from,  \MRC Human Genetics Unit / Western General Hospital
chrisb at hgu.mrc.ac.uk| not for \        Crewe Road / Edinburgh EH4 2XU / Scotland



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