antisense delivery by liposomes
Stephen R. Lasky
Stephen_Lasky at brown.edu
Thu Feb 8 08:16:03 EST 1996
Lots of replies cut out:
> > > Iddo (idoerg at shum.cc.huji.ac.il) wrote:
> > >
> > > : Hello all,
> > > : I'm in the preliminary stages of setting up an assay which requires the
> > > : delivery of phosphorothioated antisense-DNA (PS-DNA) to a primary cell-
> > > ................................^^^^^^^^^^^^^^^^^^^^^^
> > >
> > > AAAAaaaaaaarrrgggggggghhhhhhhh!!!!
> > >
> > > -Paul.
Realizing that I am one of the people opposed to the use of sense and
anti-sense for describing DNA (lets clarify that and say dsDNA especially
when refering to cistrons), I just want to say that this is the correct
use of the term. Anti-sense DNA is that same as anti-sense RNA. My
question is why modify it? I thought the advantage of using anti-sense
DNA rather than anti-sense RNA is that it is more stable and doesn't need
to be modified to be effective. The disadvantage is that the duplexes
formed are less stable than RNA:DNA duplexes. Is this wrong?
Stephen R. Lasky Ph.D. Brown U/Roger Williams Medical Center, Providence, RI.
Phone: 401-456-5672 Fax: 401-456-6569 e:mail: Stephen_Lasky at brown.edu
America may be unique in being a country which has leapt from barbarism to decadence without touching civilization. John O'Hara.
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