Puromycin Concentration for Selection in Mouse Fibroblasts?
christoph.haupenthal at uni-essen.de
Thu Feb 8 05:21:09 EST 1996
Graham Dellaire <popa0206 at PO-Box.McGill.CA> wrote:
>I am using a new Puromycin mammalian expression vector in Mouse L >fibroblasts (LTA's).... has anyone worked with these?
>I need to know the puromycin concentration to use...
>I tried 5, 10 15 and 25 micro grams/ml and they seem to be a little >high... except 5 micrograms looks promising.
I do similar experiments with the pBabePuro vector in Swiss3T3 and primary
BabyMouseKidney cells. For selection in stable transformed mouse
fibroblasts I use 2,5 microgram/ml and for primary mouse fibroblasts I use
max. 1 microgram/ml.
I hope, it works a little bit better now.
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