Stephen R. Lasky
Stephen_Lasky at brown.edu
Thu Feb 8 07:54:10 EST 1996
In article <Pine.PMDF.3.91.960205122238.564180252B-100000 at kids.wustl.edu>,
haviland at KIDS.WUSTL.EDU ("David L. Haviland, Ph.D.") wrote:
>After a few control assays, we used (and I continue to
> use) a 100mM Tris buffer pH9.5.
David, just a question on this pH. I thought the pKa for tris was a
little above 8 so it wouldn't have much buffering capacity at pH 9.5. Why
not use a buffering agent that actually buffers at pH 9.5? (I think that
glycine has a pKa at 9.6 so it should actually buffer at that pH.)
BTW, like your sig.
Stephen R. Lasky Ph.D. Brown U/Roger Williams Medical Center, Providence, RI.
Phone: 401-456-5672 Fax: 401-456-6569 e:mail: Stephen_Lasky at brown.edu
America may be unique in being a country which has leapt from barbarism to decadence without touching civilization. John O'Hara.
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