Coating-pH ?

Stephen R. Lasky Stephen_Lasky at brown.edu
Thu Feb 8 07:54:10 EST 1996


In article <Pine.PMDF.3.91.960205122238.564180252B-100000 at kids.wustl.edu>,
haviland at KIDS.WUSTL.EDU ("David L. Haviland, Ph.D.") wrote:
> 
 >After a few control assays, we used (and I continue to 
> use) a 100mM Tris buffer pH9.5.  

David, just a question on this pH.   I thought the pKa for tris was a
little above 8 so it wouldn't have much buffering capacity at pH 9.5.  Why
not use a buffering agent that actually buffers at pH 9.5?  (I think that
glycine has a pKa at 9.6 so it should actually buffer at that pH.)

BTW, like your sig.

SRLasky

-- 
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Stephen R. Lasky Ph.D.   Brown U/Roger Williams Medical Center,  Providence, RI.   
Phone: 401-456-5672     Fax: 401-456-6569     e:mail: Stephen_Lasky at brown.edu
===================================================================
America may be unique in being a country which has leapt from barbarism to decadence without touching civilization.  John O'Hara.
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^



More information about the Methods mailing list