Can seq gel sit O/N before drying?
tvasicek at watson.princeton.edu
Thu Feb 8 15:39:17 EST 1996
In article <4fb64a$n8k at crcnis3.unl.edu>, cstryker at unlinfo.unl.edu (cynthia
> That's my question! After running, is it ok for the power to go off &
> for the gel to sit overnight before fixing & staining? Thanks! Cindy at
I assume you're asking about DNA sequencing gels -- TBE/urea/polyacrylamide.
I have not seen any blurring or other problems in gels that I've had shut
off in the middle of the night. However, I don't fix or stain. My
sequencing rxns are radiolabelled so I trasfer the gel directly from the
glass plates to Whatman paper immediately after seperating the plates.
Then I cover the gel with plastic wrap and vaccuum dry it for 30 min.
My DNA seq. gels are 50% urea, 4 to 6 % acrylamide, 1x TBE.
Thomas J. Vasicek, Ph.D. tvasicek at watson.princeton.edu
Department of Molecular Biology FAX: 609/258-3345
Princeton University Phone: 609/258-2899
Princeton, New Jersey 08544
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