primer extension anyone ?
comtef at IRCM.UMontreal.CA
Thu Feb 8 15:43:39 EST 1996
On Mon, 5 Feb 1996, A. Kumar wrote:
> Date: Mon, 5 FEB 1996 15:19:31 -0600
> From: A. Kumar <akumar at blue.weeg.uiowa.edu>
> Newgroups: bionet.molbio.methds-reagnts
> Subject: primer extension anyone ?
> Hi ! I have been trying primer extension for a long time now and it has
> never worked. I am using a 28mer oligonucleotide as a primer. It labels
> well and I get a high specific activity. But it just doesn't seem to
> hybridize to the RNA. I have checked the sequence and it is correct. I
> have been using total RNA. Should I use mRNA ( poly A ), or maybe try and
> purify the RNA ? Also, if anyone could tell me a source for a good and
> reliable protocol for primer extension, I shall be grateful. Please help!
> Ashish Kumar
>>>>You could try to do a search on David A Clayton in the 1980's, he was
working on mitochondria and he mapped the origin of transcription of
a few mitochondrial DNA. In the same field (and in same years) Attardi
and Bogenhagen did similar work.
> WORKPLACE DEN
> Dept. of Anatomy, 735 Michael St. Apt 34
> College of Medicine, Iowa City
> University of Iowa, IA 52246-5523
> Iowa City, IA 52242
> Dial (319)335-7739 (319)338-0529
> e : akumar at blue.weeg.uiowa.edu
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