primer extension anyone ?

RAY-Francois Comte comtef at IRCM.UMontreal.CA
Thu Feb 8 15:43:39 EST 1996


On Mon, 5 Feb 1996, A. Kumar wrote:

> Date: Mon, 5 FEB 1996 15:19:31 -0600 
> From: A. Kumar <akumar at blue.weeg.uiowa.edu>
> Newgroups: bionet.molbio.methds-reagnts
> Subject: primer extension anyone ? 
> 
> Hi ! I have been trying primer extension for a long time now and it has 
> never worked. I am using a 28mer oligonucleotide as a primer. It labels 
> well and I get a high specific activity. But it just doesn't seem to 
> hybridize to the RNA. I have checked the sequence and it is correct. I 
> have been using total RNA. Should I use mRNA ( poly A ), or maybe try and 
> purify the RNA ? Also, if anyone could tell me a source for a good and 
> reliable protocol for primer extension, I shall be grateful. Please help! 
> Thanks.
> Ashish Kumar
> 
>>>>You could try to do a search on David A Clayton in the 1980's, he was 
working on mitochondria and he mapped the origin of transcription of 
a few mitochondrial DNA. In the same field (and in same years) Attardi 
and Bogenhagen did similar work.

Good luck

Francois       <<<<<<<

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