Trouble finding DNA concentration on Spec..

CK Wen cwen at aux.btny.purdue.edu
Fri Feb 9 14:33:52 EST 1996


oravaxcm at world.std.com (Charles A Miller) wrote:
>Hello again,
>	I am am having trouble determining DNA concentrations on a 
>spectrophotometer. When I measure the UV absorbance, I consistantly get 0 or
>even a negative number when it is obvious through gel electrophoresis of


I had this problem before when I gel-purified the DNA by using glass powder/NaI method.  After I changed to use another method to purify the
DNA fragments, I got no problem.

hope this help.

Chi-Kuang




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