Trouble finding DNA concentration on Spec..
cwen at aux.btny.purdue.edu
Fri Feb 9 14:33:52 EST 1996
oravaxcm at world.std.com (Charles A Miller) wrote:
> I am am having trouble determining DNA concentrations on a
>spectrophotometer. When I measure the UV absorbance, I consistantly get 0 or
>even a negative number when it is obvious through gel electrophoresis of
I had this problem before when I gel-purified the DNA by using glass powder/NaI method. After I changed to use another method to purify the
DNA fragments, I got no problem.
hope this help.
More information about the Methods