NTA Ni chelator for coupling?

H. David Fischer davefischer at delphi.com
Fri Feb 9 08:35:13 EST 1996

I am looking for a commercial source for the nickel chelator NTA
in form suitable for coupling to proteins.  The Ni-NTA-protein
conjugates would be used like the Qiagen Ni-NTA-alkaline phosphatase
conjugate to label recombinant proteins with 6-His affinity tags.
The NTA sold by Sigma is different from the desired compound described
originally by Hochuli.   The Hochuli NTA includes a (CH2)4 spacer and

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