RDA experience

Ella Nemeth ella at halia
Thu Feb 8 19:11:29 EST 1996

	Aloha everyone!

	We purchased Clontech's cDNA subtraction kit (which is supposed 
to be an equivalent of the RDA method). These methods are supposed to 
selectively amplify differentially expressed genes. We tried to detect 
upregulated genes in our system using Clontech's kit, but we are having 
difficulties. We don't get any selectively amplified products (PCR 
	If you have also used this kit, we would appreciate any kind of 
comments regarding your experience.

				Mahalo (thank you)

					Ella Nemeth

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