Question: lambda plaques not transparent, bacterial lawn not opaque
mikel51 at mother.com
Sat Feb 10 10:39:16 EST 1996
> >Hello Fierce Molecular Wizards --
> > I have an amplified library from Stratagene, and I suspect I am not
> >growing it correctly. The library is in lambda zap II, the host is XL1 blue
> >MRF' as recommended, and I am growing it at 37C on LB agar with LB top
> >agarose. Two unexpected things have occurred.
> > 2) The bacterial lawn is not opaque (even when the bugs are not
> >infected). There is definitely a bacterial lawn, but the bugs have not
> >nearly as thick and as opaque as they were when I grew the same bugs as
I find that it helps to use way more plating bacteria than they
recommend. You will get a much thicker lawn, and more defined plaques.
Try using 3-10X the recommended number of plating bacteria.
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