long PCRs: Yes, it works on human DNA !
popa0272 at PO-Box.McGill.CA
Sun Feb 11 16:58:22 EST 1996
I have been using inverse PCRs to determine my gene structure as an easy way to do on the side of other experiments. I am studying a human gene and for me,
some of my clones were up to 1.4-1.6 kb (I subcloned my PCR bands using the TA cloning kit). I have found about 15 intron/exon junctions by this way. I did not try to get
longer PCR products since I was interested only in the surrounding sequences (that is why I used four base cutters like NlaIII, AluI, MseI ... etc). Anyway, if some of you are
interested in the protocol we are using, e-mail me or just ask me to post it and I'll do it. Hope this will help !
e-mail: popa0272 at po-box.mcgill.ca
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