Rapid Ligation

Gary K. gtk10583 at glaxo.com
Mon Feb 12 07:49:44 EST 1996

In article <4fgj3k$4va at taco.cc.ncsu.edu>, Chip Peele <chip_peele at ncsu.edu>

> Does anyone know the secret to the Boeringer Mannheim rapid ligation 
> kit??  

I'm not so sure there is a secrete.  I typically do blunt and cohesive end
ligations at 37C for about 1 hour using my own buffers then transform.  I
nearly always get my clone.


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