HELP:Saving IEF gel

KevO kevo at astro.ocis.temple.edu
Mon Feb 12 14:31:22 EST 1996


Hi! I need some help with maintaining my mini-IEF slab gels overnite. My 
current experience with the BioRad Mini IEF is that I have to complete 
all steps (including  staining in one complete continuous process). I use 
polyacrylamide gel on gel support backing. I have experience that 
prolonged soaking of gel in either fixing or destaining solution will swell 
the gel and separate it from the backing. I would appreciate it if anyone 
has any ideas of preventing the gel from "ripping" when in fix or
destain overnite. Thanks in advance.
--
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* Kevin Ong                            * For God so loved the world that he  *
* Biology Dept.                        * gave his only begotten son...       *
* Temple University,Philadelphia       *               -JOHN 3:16            *
* O:(215)204-8843     H:(610)352-4773  ***************************************
* Fax:(215)204-6646  email> kevo at astro.ocis.temple.edu or kevo at vm.temple.edu *
* WWW URL> http://astro.temple.edu/~kevo                                     * 
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