HELP:Saving IEF gel
KevO
kevo at astro.ocis.temple.edu
Mon Feb 12 14:31:22 EST 1996
Hi! I need some help with maintaining my mini-IEF slab gels overnite. My
current experience with the BioRad Mini IEF is that I have to complete
all steps (including staining in one complete continuous process). I use
polyacrylamide gel on gel support backing. I have experience that
prolonged soaking of gel in either fixing or destaining solution will swell
the gel and separate it from the backing. I would appreciate it if anyone
has any ideas of preventing the gel from "ripping" when in fix or
destain overnite. Thanks in advance.
--
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* Kevin Ong * For God so loved the world that he *
* Biology Dept. * gave his only begotten son... *
* Temple University,Philadelphia * -JOHN 3:16 *
* O:(215)204-8843 H:(610)352-4773 ***************************************
* Fax:(215)204-6646 email> kevo at astro.ocis.temple.edu or kevo at vm.temple.edu *
* WWW URL> http://astro.temple.edu/~kevo *
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