Extracting from agarose (Re: Which kit for cleaning PCR products?)
K J C 944
kjc944 at aol.com
Tue Feb 13 00:00:52 EST 1996
An inexpensive way to clean up PCR products:
EtOH ppt then resuspend in a small volume of TE. Run out on a gel. Cut
out band and load onto the top of a P-200 aerosol resistant tip which has
been cut to fit into a microfuge tube. Spin 45 s. Discard tip, bring up
volume to 100uL, and EtOh precip using 7.5 M NH4Cl.
I've successfully cloned and sequenced PCR products that have been cleaned
up by this method.
Kathryn J. Coyne
University of Delaware
College of Marine Sciences
Lewes, DE 19958
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