primer extension

Heidi Moss hmmoss at MAIL.MED.CORNELL.EDU
Tue Feb 13 14:02:55 EST 1996


Hello 'netters!
Has anyone done primer extension using the ABI sequencer?? The technology 
seems plausible, but I cannot for the life of me come up with a protocol 
(end labelling with dye terminators, etc.) 
My gene wants to know where its transcriptional start site is! I will 
resort to the traditional method, if necessary,  but it doesn't hurt to 
inquire.
Thanks! (:
--Heidi



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