double-annealing problem

Jean.Mariaux at Jean.Mariaux at
Tue Feb 13 08:58:35 EST 1996

I am sequencing PCR products directly, using Sequenase, the method is working
very well except for a few cases where parasite  sequences mix with the desired
signal. This is apparently due to a double-annealing phenomenon. Any suggestion
as how to avoid this (except to design a new primer!). Thanks in advance.
Jean Mariaux

Jean Mariaux                         Phone:  (+41) 38 233 051
Institute of Zoology (A115)          Fax:    (+41) 38 233 001
E.-Argand 11                         E-mail: jean.mariaux at
CH-2007 NEUCHATEL                    

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