gabriel at OCELOT.RUTGERS.EDU
Tue Feb 13 11:04:27 EST 1996
We are trying to de-cap a specific yeast mRNA so that we can
subsequently ligate a linker to it's 5' end, and then perform RT-PCR.
Our hope is that we can then clone and sequence the resulting PCR
product and use this to directly determine the 5' terminal base in the
RNA. However, we have had no success with either chemical decapping
methods or enzymatic decapping using tobacco acid pyrophosphatase.
I would be interested in hearing the experiences of anyone who has
successfully used either of these methods for decapping RNA, especially
what they find to be critical factors in their success.
Dept. of Molecular Biology and Biochemistry
gabriel at mbcl.rutgers.edu
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