decapping RNA

gabriel,abram gabriel at OCELOT.RUTGERS.EDU
Tue Feb 13 11:04:27 EST 1996


We are trying to de-cap a specific yeast mRNA so that we can 
subsequently ligate a linker to it's 5' end, and then perform RT-PCR.  
Our hope is that we can then clone and sequence the resulting PCR 
product and use this to directly determine the 5' terminal base in the 
RNA.  However, we have had no success with either chemical decapping 
methods or enzymatic decapping using tobacco acid pyrophosphatase. 

I would be interested in hearing the experiences of anyone who has 
successfully used either of these methods for decapping RNA, especially 
what they find to be critical factors in their success.

Abram Gabriel
Dept. of Molecular Biology and Biochemistry
Rutgers University
908-235-5097
gabriel at mbcl.rutgers.edu






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