dml1 at columbia.edu
Tue Feb 13 17:14:26 EST 1996
I am trying to optimize a hemi-nested DNA PCR protocol. My problem is
smearing of lanes on the resulting gels with certain of the primers.
Others give crisp bands.
I have tried temperature otpimization, have cut back on dNTP and TAQ
concentrations and have even added DMSO. Still smearing with occasional
bands buried in the smear. The DNA sample amplifies well with other
primers. Any suggestions?
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