Lambda DNA prep.

john brennand john.brennand at gbapr.zeneca.com
Wed Feb 14 05:14:42 EST 1996


One trick we have always used is to mix the phage lysates (plate or 
liquid) 50:50 with a DE52/TE slurry, shake, spin to remove slurry, 
repeat x2, then continue with SDS/PK, Etoh ppt.  This effectively takes 
out all the c*** from the lysate that if carried over will inhibit 
restriction enzymes etc.

Never fails





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