Lambda DNA prep.

john brennand john.brennand at
Wed Feb 14 05:14:42 EST 1996

One trick we have always used is to mix the phage lysates (plate or 
liquid) 50:50 with a DE52/TE slurry, shake, spin to remove slurry, 
repeat x2, then continue with SDS/PK, Etoh ppt.  This effectively takes 
out all the c*** from the lysate that if carried over will inhibit 
restriction enzymes etc.

Never fails

More information about the Methods mailing list