polishing PCR fragments with T4 Pol ?

Burkhard Hassel bhassel at orion.rz.mdc-berlin.de
Tue Feb 13 13:38:20 EST 1996


Dear Netters,

Blunting PCR fragments with T4 DNA polymerase is often prefered from that with
Klenow.
My question is: has anybody tested this at room temperature rather than the 11
or 15 degrees mentioned in several protocols ?
-Just for convenience.

Burkhard Hassel (bhassel at orion.rz.mdc-berlin.de)



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