polishing PCR fragments with T4 Pol ?

Frances Hannan Zoology flh at mole.bio.cam.ac.uk
Wed Feb 14 08:13:40 EST 1996


bhassel at orion.rz.mdc-berlin.de (Burkhard Hassel) writes:

>Blunting PCR fragments with T4 DNA polymerase is often prefered from that with
>Klenow.
>My question is: has anybody tested this at room temperature rather than the 11
>or 15 degrees mentioned in several protocols ?

I do it at 30oC routinely.


-- 
Frances Hannan                                       
flh at mole.bio.cam.ac.uk                               
"I feel like a mosquito in a nudist camp - I know what I ought to do, but 
I don't know where to begin..."



More information about the Methods mailing list