NATIVE GEL ELECTROPHORESYS ANSWER

ming at msvax.mssm.edu ming at msvax.mssm.edu
Wed Feb 14 12:24:37 EST 1996


I am not sure whAt you are talking about.
We currently running 5-6 native gels per day and our results are super.
I guess you have to makre sure your proteins are running under the right
native system. Detergent is not necessary in most valid cases.

In article <199602130042.QAA01489 at net.bio.net>, spagnol at GALACTICA.IT (Giorgio Spagnol) writes:
>I WOULD LIKE TO KNOW SOMEBODY WHO SUCCESSFULLY DID ONE.
>IT SIMPLY DOES NOT WORK.
>HOWEVER, IF YOU ARE WILLING TO TRY ANOTHER METHOD, YOU COULD USE 
>ZWITTERIONIC DETERGENTS TO PARTIALLY RECONSTITUTE YOUR PROTEIN AFTER SDS 
>DENATURATION AND BEFORE GEL LOADING.THE REFERENCE IS IN CURRENT 
>PROTOCOLS OF MOLECULAR BIOLOGY, BY WILEY AND SONS, NEW YORK, BUT, IF YOU 
>DO NOT HAVE THE BOOK, I WILL THE REFERENCE TO YOU, AS SOON I WILL RETURN 
>TO MY LAB.
>CORDIALLY, 
>GIORGIO SPAGNOL
>
>
>
>



More information about the Methods mailing list