DH5a in DMEM

john brennand john.brennand at gbapr.zeneca.com
Thu Feb 15 04:39:49 EST 1996

I hesitate  to ask why you want to use expensive DMEM... but...

Are you sure someone hasn't added a vial of Pen/Strep to the DMEM :-)

That seems to be standard practice in our lab !

If not just check the genotype of DH5a and see if it "requires" anything 
that may be absent, eg, Thiamine, Thymine - everything else should be 


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