Partial RE digestion - please advise!
mantei at neuro.biol.ethz.ch
Thu Feb 15 07:10:25 EST 1996
In article <U10784.15.0011F447 at UICVM>, U10784 at UICVM (Victor Levenson) wrote:
Today I tried digestion in the presence of EtBr -
> works great for DNaseI - and I could not get ANY cuts!
You have to try a range of EtBr concentrations (5, 10, 20, 40, 80, 160
micrograms per ml). I would use about 1 unit enzyme per microgram DNA,
incubate about 30 minutes.
Dept. of Neurobiology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland
mantei at neuro.biol.ethz.ch Fax: +41-1-633-1046
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