pfu vs taq

Darren Boehning boehnin1 at
Fri Feb 16 16:12:01 EST 1996

We have found that pfu does work more slowly, and as a result we do an
extension of about 2-3 minutes per kb.  This generally results in very
good amplification that is (percievedly) relatively mutation free for 4-5
kb (we have not attempted more, but the Stratagene people tell me they
routinely go up to and beyond 10kb).  We have also had good success with
the Extend system from BMB

Darren Boehning
Thomas Jefferson University
boehnin1 at

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